Description
The ViPrimePLUS Pseudomonas aeruginosa qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of the Pseudomonas aeruginosa genomes in clinical samples (e.g. blood, cerebrospinal fluid and respiratory samples) and food or water samples.
Pseudomonas aeruginosa is a Gram negative, opportunistic human pathogen and plant pathogen. The microorganism is characterized by its pigment production ability, such as pyocyanin (blue-green), fluorescein (yellow-green and fluorescent, now also known as pyoverdin) and pyorubin (red-brown), and its fruity odor in culture. Infection with P. aeruginosa in immunocompromised patients causes pneumonia, central nervous and urinary tract infections, and bacteremia. It is responsible for about 10% of all hospital acquired infection, making it an important nosocomial pathogen. Transmission of the infection is by ingesting contaminated food and water, or via direct contact with infected patients.
ViPrimePLUS Pseudomonas aeruginosa qPCR Kit is intended for research use only.
Kit Component
- P. aeruginosa Primers and Probe Mix (P. aeruginosa PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | • Clinical samples (e.g. blood, cerebrospinal fluid, respiratory samples) • Food samples • Water samples |
| Gene Target | Toxin A synthesis regulating gene (RegA) |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2048 | ViPrimePLUS Pseudomonas aeruginosa qPCR Kit | 150 reactions |
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