Description
The ViPrimePLUS Treponema pallidum qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Treponema pallidum genomes in clinical samples (e.g. genital fluids and swabs).
Treponema pallidum is a Gram negative, spirochaete bacterium which causes syphilis. It is usually transmitted via sexual intercourse, from mother to infant (which causes congenital infection), and sometimes through blood transfusion. Primary stage infection can be observed by the formation of syphilis sores on the external genital areas, which then progressively develops into the secondary stage where the development of rashes in other parts of body, sore throat, fever, headaches and hair loss are observed. Tertiary stage can appear after 10-30 years of primary infection resulting in neurological illness, multi-organ damages, optical and muscular deterioration. Previous syphilis infection does not provide immunity; however, treatment with penicillin is effective. Many developing countries are scaling-up the screening program for pregnant ladies to reduce congenital syphilis cases which is a major cause of stillbirth and death of young infants.
ViPrimePLUS Treponema pallidum qPCR Kit is intended for research use only.
Kit Component
- T. pallidum Primers and Probe Mix (T. pallidum PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. genital fluids and swabs) |
| Gene Target | polA gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2065 | ViPrimePLUS Treponema pallidum qPCR Kit | 150 reactions |
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