Description
The ViPrimePLUS Acanthamoeba qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of all Acanthamoeba species genomes in clinical samples (e.g. cerebrospinal fluid).
Acanthamoeba is one of the few free-living amoebas, including Naegleria, Balamuthia mandrillaris and Sappinia diploidea that cause central nervous system (CNS) infections in human. As there are no human carrier state and insect vector, the pathogen is acquired from environment with poor sanitation, which enters via breaks in the skin epithelial layer or inhalation of the cysts. It commonly causes amebic keratitis, usually among those contact lens wearers, which can be detrimental to the sight if left unattended. Prognosis for the disease is good when treated early. In more severe, rare cases, granulomatous amebic encephalitis (GAE) and disseminated granulomatous amebic disease (eg, skin, sinus, and pulmonary infections) may occur in immunocompromised patients.
ViPrimePLUS Acanthamoeba qPCR Kit is intended for research use only.
Kit Component
- Acanthamoeba Primers and Probe Mix (Acanthamoeba PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. cerebrospinal fluid). |
| Gene Target | 18S ribosomal RNA (18S) |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • Internal Extraction Control (IEC) as inhibition control • Positive control |
Ordering Information
| Catalog No | Description | Pack Size |
| QM1001 | ViPrimePLUS Acanthamoeba qPCR Kit | 150 reactions |
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