Description
The ViPrimePLUS Entamoeba qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of all Entamoeba species genomes in clinical samples (e.g. faeces) and food or water samples.
Entamoeba protozoa are usually found in the large intestines of animals and humans, except E. gingivalis which resides in the mouth as a commensal. One important human pathogenic species is E. histolytica, which causes amebiasis. Most cases are asymptomatic, but bowel inflammation, dysentery and rare incidences of invasive extraintestinal disease may occur. Invasive amebiasis may result in multiorgan damage, such as amebic liver abscess due to the spread of the trophozoites in the circulatory system. Transmission is via fecal-oral route, by ingesting the cysts in contaminated food or water. Amebiasis is the fourth leading cause of death due to protozoan infections worldwide, resulting in ~70,000 deaths annually, with cases reported mostly from areas with poor sanitation.
ViPrimePLUS Entamoeba qPCR Kit is intended for research use only.
Kit Component
- Entamoeba Primers and Probe Mix (Entamoeba PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | • Clinical samples (e.g.faeces)• Food samples• Water samples |
| Gene Target | 18S ribosomal RNA (18S) gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM1003 | ViPrimePLUS Entamoeba qPCR Kit | 150 reactions |
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