Description
The ViPrimePLUS Naegleria qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Naegleria genomes in clinical samples (e.g. brain tissue and cerebrospinal fluids).
Naegleria spp. is one of the free-living amoebas that cause primary amoebic meningoencephalitis (PAM) in humans. Out of more than 30 species discovered, only N. fowleri is associated with human disease. Being a thermophilic organism, Naegleria are found in soil and warm freshwater source including swimming pools, hot springs, sewage, domestic water supplies and thermally polluted water from power plant. Unlike the Acanthamoeba and Balamuthia, N. fowleri is not an opportunistic pathogen as it causes acute, fulminant, necrotizing and hemorrhagic meningoencephalitis leading to death in healthy children and young adults. The onset of symptoms is rapid following the exposure, starting with the loss of smell, nausea and seizures. Infected individuals often die from respiratory failure as the infection spreads to the brain stem after around two weeks. Poor prognosis is mainly due to the late diagnosis and treatment.
ViPrimePLUS Naegleria qPCR Kit is intended for research use only.
Kit Component
- Naegleria Primers and Probe Mix (Naegleria PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. brain tissue, cerebrospinal fluids) |
| Gene Target | 18S ribosomal RNA (18S) gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM1005 | ViPrimePLUS Naegleria qPCR Kit | 150 reactions |
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