Description
M-MuLV Reverse Transcriptase III is an upgraded version of M-MuLV Reverse Transcriptase RNase H- which can perform highly efficient reverse transcription reactions at 37°C - 55°C. The enzyme is from recombinant E.coli containing the modified pol gene of Moloney Murine Leukemia Virus (M-MuLV). The reverse transcription temperature can be increased to 50°C - 55°C, avoid the inhibition of cDNA synthesis by RNA complex secondary structure and can effectively synthesize high-quality cDNA. It can generate cDNA from 100bp to >12 kb. M-MuLV Reverse Transcriptase III has superior continuous synthesis ability and super strong impurity tolerance.
Concentrations
200u/μl
Supplied With 5X Buffer M-MuLV
250mM Tris-HCl, 375mM KCl, 15mM MgCl2 and 50mM DTT
Storage Buffer
20mM Tris-HCl, 100mM NaCl, 0.1 mM EDTA, 1mM DTT, 50% (v/v) glycerol
Thermal Inactivation
85˚C for 5 seconds
Unit Definition
85˚C for 5 seconds 1u is defined as the amount of the enzyme that is required to incorporate 1nmol of dTTP into an acid-insoluble material in 10 minutes at 37°C using poly(rA)•oligo (dT).
Application
- First strand cDNA synthesis.
- DNA labeling.
- RNA analysis by primer extension.
Quality Control
Purified free of detectable levels of RNase, endonucleases and exonucleases activities
Ordering Information
| Catalog No | Description | Pack Size |
|---|---|---|
| ME2307 | M-MuLV Reverse Transcriptase III | 10000u |
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