Description
Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase is an RNA dependent DNA polymerase. It can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template. The absence of RNase H activities enhances the synthesis of long cDNAs and therefore the enzyme is recommended for preparing long cDNAs.
Concentrations
100 - 500u/μl
Assay Conditions
50mM Tris- HCl (pH8.3), 6mM MgCl2, 10mM DTT, 0.4mM Poly(rA)•(dT)12-18, and 0.5mM [ 3H]dTTP in a reaction volume of 50μl./μl
Supplied With
10X Buffer M-MuLV RT
500mM Tris-HCl (pH 8.3 at 25˚C), 75mM KCl, 3mM MgCl2 and 10mM DTT. Store at -20˚C.
Storage Buffer
10mM K-Phosphate (pH 7.5), 0.1mM EDTA, 200mM NaCl, 7mM 2-mercaptoethanol and 50% glycerol. Store at -20˚C.
Thermal Inactivation
70˚C for 10 minutes
Unit Definition
1u is defined as the amount of enzyme that is required to incorporate 1nmol of dTTP into an acid-insoluble material in 10 minutes at 37˚C using Poly(rA)•oligo(dT).
Application
- First strand cDNA synthesis.
- DNA labeling.
- RNA analysis by primer extension.
Quality Control
Purified free of contaminating endonucleases and exonucleases.
Ordering Information
| Catalog No | Description | Pack Size |
|---|---|---|
| ME2305 | M-MuLV Reverse Transcriptase (RNase H¯) | 10000u |
| ME2306 | M-MuLV Reverse Transcriptase (RNase H¯) | 50000u |
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Manual
M-MuLV Reverse Transcriptase (RNase H¯)
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MSDS
M-MuLV Reverse Transcriptase (RNase H¯)
Publication
This Product Has Been Used In:
Atasoy M O et al. (2022) Genetic diversity, frequency and concurrent infections of picobirnaviruses in diarrhoeic calves in Turkey, Tropical Animal Health and Production , 54:127
Nazaktabar, Ahmad et al. (2022). Molecular typing of group A bovine rotavirus of calves in the provinces of Tehran, Alborz and Qazvin. Iranian Journal of Veterinary Research. 10.22059/ijvm.2012.30221.
Wiriyaampaiwong P et al. (2022) Cathelicidin-HR from Hoplobatrachus rugulosus: an antioxidant peptide that performs a protective effect against UV/H2 O2 -induced DNA damage. Journal of Applied Biology & Biotechnology Vol. 10(4), pp. 35-44, Jul-Aug, 2022
(2020) Detection of Tomato Mosaic Virus Viruses in Farms and Greenhouses and Breed Several Isolates of The Potato Virus Y From this Host International Journal of Advanced Science and Technology, 29(8s):1650-1655.
Faezebossadat A & Seyyedeh AH (2020) Incidence and distribution of Tobacco streak virus isolated from parsley Petroselinum sativum, Journal of Crop Protection, 9(4): 683-697.
Mirzaei et al. (2019) Synergistic effects of polyaniline and pulsed electromagnetic field to stem cells osteogenic differentiation on polyvinylidene fluoride scaffold, . Artificial Cells, Nanomedicine and Biotechnology,47(1):3058-3066.
Moradi, N. et al. (2019) Plasma Level of miR-5193 as a Novel Biomarker for Diagnosis of HBV-Related Hepatocellular Carcinoma, . Hepatitis Monthly, 19(2):e84455.
Kaplan, S. et al. (2016) The Potential of Microarray Databases to Identify Tissue Specific Genes Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 22(1), p. 29-35.
Noureini, S.K., Tanavar, F. (2015) Boldine, a natural aporphine alkaloid, inhibits telomerase at non-toxic concentrations. Chemico-Biological Interactions. 231. Pp27-34.
Tahoori, M.T., Pourfathollah, A.A., Soleimani, M., Vasheghani-Farahani, E., Mohammadzadeh, A., Amari, A., Hashemi, S.M., mossahebi-mohammadi, M. (2015) Fibroblasts feeder niche and Flt3 Ligand as a novel inducer of plasmacytoid dendritic cells development in vitro. International Immunopharmacology. 24. Pp.474-480.
Ahmadi, E., Ahmadi, M., PourBahsh, S.A., Talebi, A. (2013). Detection and Differentiation of Newcastle Disease Virus Strains Affecting Commercial Poultry in Northwest of Iran using RT-PCR, International Journal of Veterinary Science, Vol 2, No. 4, 138-142.
Supathaweewat, K., Klanrit, P (2013). cDNA cloning and expression analyses of phytoene synthase 1, phytoene desaturase and -carotene desaturase genes from Solanum lycopersicum KKU-T34003, Songklankarin Journal of Science and Technology, Vol. 35, No. 5, 517-527 (2013).
