Storage
Store at -20°C
Grade
Ultra Pure Grade
Description
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N’-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.
Specification
Appearance | White powder |
Mol. Formula | C4H10O2S2 |
Mol. Weight | 154.25 |
CAS RN | 3483-12-3 |
Assay | Specifications |
Titration ex –SH (iodometric) | ≥99.0% |
T.L.C. | One spot |
UV Assay | |
1% Solution A280 | <0.067 |
A305 | <0.050 |
0.02M Solution A283 | <0.040 |
1M Neutral Solution A400 | <0.062 |
A405 | <0.062 |
OD 700-500nm | ≤0.035 |
Loss on Drying | <0.5% |
Melting Point | 42±2°C |
Oxidized DTT | <0.5% |
Solubility (10% Water) | Clear and colorless |
I.R. | Consistent with assigned structure |
DNase | None |
RNase | None |
Protease | None |
Ordering Information
Catalog No | Description | Pack Size |
PC0705 - 5g | DL-Dithiothreitol (DTT) | 5g |
PC0705 - 25g | DL-Dithiothreitol (DTT) | 25g |
PC0705 - 100g | DL-Dithiothreitol (DTT) | 100g |
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