Grade
Molecular Biology Grade
Description
RNase A is an endoribonuclease that is from bovine pancreas for molecular biology applications. The major application for RNase A is the removal of RNA from preparation of plasmid DNA as well as extraction of plasmid DNA. It is also used in removal of unspecifically bound RNA; RNase protection assays; analysis of RNA sequences as well as hydrolysis of RNA contained in protein samples.
RNase A attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. RNase A can be inhibited by alkylation of His12 or His119, which are present in the active site of the enzyme. Activators of RNase A include potassium and sodium salts.
Source
Bovine pancreas
All products from animal sources are produced from starting material of USDA-approved origin, collected in United States Department of Agriculture (USDA) or equivalent approved facilities, inspected to be free of disease and suitable for exportation. Certificates of Origin are available upon request.
Unit Definition
One unit according to Kunitz produces an increase in absorbance of 0.001/minute under assay conditions in 1ml at 260nm.
Specification
| Assays | Results |
| Activity (Kunitz) | ≥60 units/mg protein |
| Molecular mass | 13.7 kDa (amino acid sequence) |
| Extinction coefficient | E1% = 7.1% (280nm) |
| Isoelectric point | pI: 9.6 |
| Optimum temperature | 60°C (activity range of 15 - 70°C) |
| Optimum pH | 7.5 (activity range of 6 - 10) |
| Inhibitors | Ribonuclease inhibitor |
| Loss on drying | ≤5.0% |
| DNase | None detected |
| Solubility (1% water) | Pass |
| Chloride | ≤0.014% |
| Sulfate | ≤0.15% |
| Heavy metals | ≤0.002% |
| Arsenic | ≤8ppm |
| Aluminium, Ca & related elements | Pass |
| Residual solvents | Pass |
Notes
For the application of removal of RNA from preparations of plasmid DNA, the suggested final concentration used is 0.2 mg/mL. Boiling stock solutions of the RNase A to inactivate residual DNase I is not necessary and may cause precipitation of RNase A and possible loss of enzymatic activity. If the RNase A solution is heated at a neutral pH, precipitation will occur. If the RNase A solution heated at a lower pH, some precipitation may occur due to the protein impurities that are present.
Storage
Store at -20°C
Ordering Information
| Catalog No | Description | Pack Size |
| PC0713-250mg | Ribonuclease A (RNase A) | 250g |
| PC0713-500mg | Ribonuclease A (RNase A) | 500mg |
| PC0713-1g | Ribonuclease A (RNase A) | 1g |
| PC0713-1ml | Ribonuclease A (RNase A) | 1ml |
Download
Manual
Ribonuclease A (RNase A) (lyophilized form)
Ribonuclease A (RNase A) (solution form)
Download
MSDS
Publication
This Product Has Been Used In:
Poon N K. et al. (2019) Optimization of CTAB-based RNA Extraction for in planta Fusarium oxysporum f. sp. cubense Gene Expression Study. Sains Malaysiana, 48(10):2125–2133.
Abdel-Gawad, F.K. et al. (2014) Carboxymethyl Chitosan Modulates the Genotoxic Risk and Oxidative Stress of Perfluorooctanoic Acid in Nile Tilapia (Oreochromis niloticus). Journal of the Saudi Society of Agricultural Sciences.ScienceDirect.
