GF-1 Soil Sample DNA Extraction Kit

Description
The GF-1 Soil Sample DNA Extraction Kit is designed for the rapid and efficient purification of bacteria DNA from soil samples without the need for precipitation or organic extraction. The kit uses a high pure specially-treated silica-based material fixed into a column to efficiently bind DNA in the presence of high salt. The kit applies the principle of a minicolumn spin technology and the use of optimized buffers to ensure that only DNA is isolated while cellular protein, humic acid, metabolites, salts and other low molecular weight impurities are removed during the subsequent washing steps. High quality DNA eluted in low salt buffer or water is ready to use in downstream applications such as restriction enzyme digestion, PCR and other manipulations.

Features

No organic-based extraction required.
Highly pure DNA ready to use for routine molecular biology applications such as PCR.

Kit Components

GF-1 DNA Binding Columns
Collection tubes
Glass Beads
Buffer SL1
Buffer SL2
Buffer SB
SPW Wash Buffer 1
SPW Wash Buffer 2
Elution Buffer
Handbook

Figure: Gel photos show genomic DNA extracted from different soil samples using old version of GF-1 Soil Sample DNA Extraction Kit (left) and improved version of GF-1 Soil Sample DNA Extraction Kit (right). The improved version of Soil Sample Extraction Kit extracts genomic DNA with better yield as well as better purity (refer to OD reading). The improved version of kit has shorten the extraction time with no organic-based extraction required

OD reading (gel photo-left)
concentration		260/280	260/230
Sample A	1.79ng/ul	2.90	0.24
	1.82ng/ul	2.91	0.21
Sample B	5.50ng/ul	2.21	1.05
	5.77ng/ul	2.20	1.05
Sample C	2.66ng/ul	1.83	1.56
	2.89ng/ul	1.85	1.54
Sample D	6.50ng/ul	2.03	1.35
	6.66ng/ul	2.00	1.34

OD reading (gel photo-right)
concentration		260/280	260/230
Sample A	7.9ng/ul	2.07	0.21
	7.35ng/ul	2.07	0.22
Sample B	20.72ng/ul	1.87	1.34
	20.56ng/ul	1.80	1.35
Sample C	16.74ng/ul	1.77	1.19
	17.16ng/ul	1.80	1.19
Sample D	34.50ng/ul	1.75	0.47
	33.21ng/ul	2.01	0.44