Description
The GF-1 Tissue DNA Extraction Kit is designed for rapid and efficient purification of genomic DNA from various tissue samples such as kidney, heart, lungs, brain, muscles, liver, spleen, and animal cultured cells. The purification is based on the usage of denaturing agents to provide lysis of cells, denaturation of proteins and subsequently release of genomic DNA. Special buffers provided in the kit are optimized to enhance binding of DNA onto a specially-treated glass filter membrane for efficient recovery of highly pure genomic DNA.
Features
- Yields up to 20μg of DNA
- No organic-based extraction required.
- Highly pure genomic DNA ready to use for routine molecular biology applications such as restriction enzyme digestion, PCR, Southern blotting and DNA fingerprinting.
Kit Components
- Buffer TL
- Lysis Enhancer
- Buffer TB
- Wash Buffer (concentrate)
- Elution Buffer
- Proteinase K
Ordering Information
Catalog No | Description | Pack Size |
GF-TD-050 | GF-1 Tissue DNA Extraction Kit | 50 preps |
GF-TD-100 | GF-1 Viral Nucleic Acid Extration Kits |
100 preps |
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Manual
GF-1 Tissue DNA Extraction Kit
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MSDS
GF-1 Tissue DNA Extraction Kit
Stability Test Report
GF-1 Tissue DNA Extraction Kit
Publication
This Product Has Been Used In:
Zeki A et al. (2022) Salmonella Detection in Different Types of Packed Raw Poultry Meat by Culture ELISA and PCR methods, Fresenius Environmental Bulletin Vol 31-No.01A/2022 pages 1531-1536
P. Rachprakhon & W Purivirojkul (2021) Very low prevalence of Opisthorchis viverrine s.l. cercariae in Bithynia siamensis siamensis snails from the canal network system in the Bangkok Metropolitan Region, Thailand, Parasite, 28 (2).
Hani G Keshta et al. (2020) Detection of lumpy skin disease during an outbreak in summer 2019 in Menoufia Governorate, Egypt using clinical, biochemical and molecular diagnosis, Zagazig Veterinary Journal, 48(4):378-389.
Lima et al. (2020) A biogeographic framework of octopod species diversification the role of the Isthmus of Panama, PeerJ, 8:e8691.
Patarwut, L. et al. (2020) Infections of Digenetic Trematode Metacercariae in Wrestling Halfbeak, Dermogenys pusilla from Bangkok Metropolitan Region in Thailand, . The Korean Journal of Parasitology, Vibrio vulnificus Isolates, Antibiotics, 58(1):27-35.
Abd El-Razik, K.A.E. et al. (2019) Species-specific PCR test for the quick recognition of equine tissue in raw and processed beef meat mixtures, . Food Science and Technology, 39:166-172.
Gholami, E. et al. (2019) DNA plasmid coding for Phlebotomus sergenti salivary protein PsSP9, a member of the SP15 family of proteins, protects against Leishmania tropica, . PLoS Neglected Tropical Diseases, 13(1): e0007067.
Mohamad Ikbal Nurul Huda et al. (2019) Prevalence and genetic divergence of Leptospira interrogans and Leptospira borgpetersenii in house rats (Rattus rattus) from Peninsular Malaysia,. Asian Pacific Journal of Tropical Medicine, 12(10):463-471.
Wiroonpan P. & Purivirojkul W. (2019)New record of Trichodina unionis (Ciliophora, Trichodinidae) from freshwater gastropods in Bangkok, Thailand . Parasite, 20 (47).
Malaitad, T., Poeaim, S., Eiamampai, K. (2015). Sex identification in barn swallows (Hirundorustica Linnaeus) by molecular technique, Journal of Agricultural Technology, Vol. 11, No. 8, 2411-2418 (2015).
Abdossamadi, Z., Seyed, N., Zahedifard, F., Taheri, T., Taslimi, Y. (2017) )Human Neutrophil Peptide 1 as immunotherapeutic agent against Leishmania infected BALB/c mice. . PLOS Neglected Diseases Vol. 11, No. 12 (2017).
Ghatar, R.H., Soltantoyeh, T., Bahadori, T., Golara. M., Hassania, H., Eghbal, R.K., Khoshnoodi, J., Judaki, M.A., Golsaz-Shirazi, F., Jeddi-Tehrani, M., Amiri, M.M., Shokri, F., (2017) Epitope Mapping of Human HER2 Specific Mouse Monoclonal Antibodies Using Recombinant Extracellular Subdomains. Asian Pacific Journal of Cancer Prevention Vol 18 DOI:10.22034/APJCP.2017.18.11. 31033
Waiho, K., Fazhan, H., Glenner, H., Ikhwanuddin, M. (2017). DInfestation of parasitic rhizocephalan barnacles Sacculina beauforti (Cirripedia, Rhizocephala) in edible mud crab, . Scylla olivacea, PeerJ (2017).
Abbas, B.M.R. (2016). Molecular detection of Helicobacter pylori among gastroduodenitis and peptic ulcer patients in Khartoum state. Journal of Microbial and Biochemical Technology8 (6) pg. 73.
Kim, Y.N., Dickinson, N., Bowie, M., Robinson, B., Boyer, S. (2017). Molecular identification and distribution of native and exotic earthworms in New Zealand human-modified soils, New Zealand Journal of Ecology, Vol 41, No. 23 (2017).
Alabsi, A.M., et al. (2016) Cell Cycle Arrest and Apoptosis Induction via Modulation Mitochondrial Integrity by Bcl-2 Family Members and Caspace Dependence in Dracaena cinnabari-Treated H400 Human Oral Squamous Cell Carcinoma. BioMed Research International.
Chontananarth, T. (2016) Multiplex PCR assay for discrimination of Centrocestus caninus and Stellantchasmus falcatus. Asian Pacific Journal of Tropical Biomedicine.
Chuah,L.O., He, X.B., Effarizah, M.E., Syahariza, Z.A., Shamila-Syuhada, A.K., Rusul, G. (2016) Mislabelling of beef and poultry products sold in Malaysia. Food Control. 62. Pp157-164
Abdo el Motalab, Y.S., & Ahmed, A.B. (2014) Isolation and Identification of Camelpox Virus in Eastern Sudan SUST Journal of Agricultural and Veterinary Sciences (SJAYS). 15(2), p. 73-81.
Chontananarth, T., Wongsawad, C., Chomdej, S., Krailas, D., Chai, J.Y. (2014) Molecular phylogeny of trematodes In Family Heterophyidae based on mitochondrial cytochrome c oxidase subunit I (mCO I). Asian Pacific Journal of Tropical Medicine. Pp..446-450.
Noikong, W., Wongsawad, C. (2014) ) Epidemiology and molecular genotyping of echinostome metacercariae in Filopaludina snails in Lamphun Province, Thailand. Asian Pacific journal of tropical Medicine. 7(1). Pp.26-29.
Ayaz, S., Islam, N.M.A.E., Rubab, L., Ullah, R., Khan, S., Hussain, R. (2013). Prevalence and Molecular Detection of Giardia and Cryptosporidium spp in Communities Consuming Different Drinking Water Sources in Kohat, Khyber Pakhtunkhwa Pakistan, Life Science Journal, Vol. 10, No. 1 (2013).
Sade, A., & Biun, H. (2009)Detection of Pig Residue in Fish Feedstuffs and Fish Stomach Content Using Polymerase Chain Reaction for Halal Certification Proceedings of the 5th National Fisheries Symposium 2008: Kuala Terengganu (Malaysia), 2008: 143-148