Vivantis Technologies Sdn Bhd

Concentration
10-20u/μl

5'…CC↓WGG…3'
3'…GGW↑CC…5'

Reaction Conditions
1X Buffer V5
30mM Tris-acetate (pH7.9 at 30°C, 10mM Mg-acetate, 60mM K-acetate, and 100µg/ml BSA. Incubate at 60°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
None.

Ligation / Recutting Assay
After 2-fold overdigestion with Bst2UI, none of the DNA fragments can be ligated.

Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of Bst2UI for 16 hours at 60°C.

Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

Download
Manual
Bst2UI {EcoRII*}

Download
MSDS
Bst2UI {EcoRII*}