Vivantis Technologies Sdn Bhd

Description
ViPrimePLUSHigh 4X One Step RT-qPCR Green Master Mix I is the improved version of master mix working for one-step amplification of any RNA template including total RNA, mRNA and etc using real-time PCR method.

ViPrimePLUSHigh 4X One Step RT-qPCR Green Master Mix I is improved to have 4X concentration and consists of improved technology of enzyme of Reverse Transcriptase and Hot Start Taq DNA Polymerases; dNTPs/dUTP Mix, RNase Inhibitor and Heat-labile UDG that allow less contaminated reaction preparation as well as leads to increased performance in sensitivity and specificity. This master mix is having SYBR® Green dye emits fluorescence when bound to doublestranded DNA without probes. Detection of PCR product is monitored by the increase in fluorescence.

Separated tubes of ROX™ dye are provided to allow the master mixes to use with most of the real-time PCR instruments. The master mixes can be used for fast cycling as suggested for any qualitative tests.

Applications
All kinds of RNA sample material suited for RT-qPCR amplification can be used.

Features

• One step reaction set up for real-time PCR or conventional PCR
• Equipped with improved generation of thermostable Reverse Transcriptase and Hot Start Taq DNA Polymerases
• Includes SYBR® Green dye for intercalator-based qPCR
• Optimal performance for highly sensitive and specific RT-PCR reaction compared to QLMM04
• Compatible with most of the real-time PCR platforms

Component

• 1ml Nuclease-free Water
• 0.5ml ViPrimePLUSHigh 4X One Step RT-qPCR Green Master Mix I
• 0.5ml LOW ROX™ Dye
• 0.5ml HIGH ROX™ Dye

Storage
Stable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.

Quality Control
As part of the ISO9001:2015 quality assurance systems, each lot of ViPrimePLUSHigh 4X One Step RT-qPCR Master Mix has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.

Limitation Of Use
For research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.

Instruments
To calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.

Ordering Information

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Manual

ViPrimePLUSHigh 4X One Step RT-qPCR Green Master Mix I