Description
ViPrimePLUSHigh 5X One Step RT-qPCR Master Mix is the improved version of master mixes working for one-step amplification of any RNA template including total RNA, mRNA and etc using real-time PCR method or conventional PCR method.
ViPrimePLUSHigh 5X One Step RT-qPCR Master Mix is improved to have 5X concentration and consists of improved technology of enzyme of Reverse Transcriptase and Hot Start Taq DNA Polymerases; dNTPs/dUTP Mix, RNase Inhibitor and Heat-labile UDG that allow less contaminated reaction preparation as well as leads to increased performance in sensitivity and specificity.
Separated tubes of ROX™ dye are provided to allow the master mixes to use with most of the real-time PCR instruments. The master mixes can be used for fast cycling as suggested for any qualitative tests.
Applications
All kinds of RNA sample material suited for RT-qPCR amplification can be used.
Features
- One step reaction set up for real-time PCR or conventional PCR
- Equipped with improved generation of thermostable Reverse Transcriptase and Hot Start Taq DNA Polymerases
- Optimal performance for highly sensitive and specific RT-PCR reaction compared to QLMM03
- Compatible with most of the real-time PCR platforms
Component
- 1ml Nuclease-free Water
- 0.4ml ViPrimePLUSHigh 5X One Step RT-qPCR Master Mix
- 0.5ml LOW ROX™ Dye
- 0.5ml HIGH ROX™ Dye
Storage
Stable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.
Quality Control
As part of the ISO9001:2015 quality assurance systems, each lot of ViPrimePLUSHigh 5X One Step RT-qPCR Master Mix has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.
Limitation Of Use
For research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.
Instruments
To calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.
| Master Mixes | Compatible Hardware |
| ViPrimePLUSHigh 5X One Step RT-qPCR Master Mix | Analytik Jena qTower series, BioRad iCycler all series, BioRad CFX96 & CFX384, Cepheid SmartCycler®, Eppendorf Mastercycler series, Fluidigm BioMark™, Illumina Eco, MJ Chromo4, Opticon, PCRMax Eco™, Roche lightcycler® series, Qiagen RotorGene, Thermo PikoReal™ |
|
ViPrimePLUSHigh 5X One Step RT-qPCR Master Mix with Low ROX |
Agilent / Stratagene MX MX3000P®, MX3005P®, MX4000®, Applied Biosystems 7500 and 7500 FAST platform, QuantStudio™, ViiA7 |
| ViPrimePLUSHigh 5X One Step RT-qPCR Master Mix with High ROX | Applied Biosystems 7000,7300,7700,7900 and 7900HT FAST platforms, OpenArray PRISM 7000,7700,7900, GeneAmp® 5700, StepOne™, StepOne™ PLUS |
Ordering Information
| Catalog No | Description | Pack Size |
| QLMM09 | ViPrimePLUSHigh 5X One Step RT-qPCR Master Mix | 100 reactions |
Download
Manual
ViPrimePLUSHigh 5X One Step RT-qPCR Master Mix
Comparison Test of RNA Sample using ViPrimePLUS One Step AtTaq RT-qPCR Master Mix & ViPrimePLUSHigh 5X One Step RT-qPCR Master Mix (Improved Version)
