Description
ViPrimePLUS AtTaq qPCR Green Master Mix I is next generation first choice mix designed for fast and easy real-time PCR reaction set up. The improved formulation of master mix contains pure Hot Start Taq DNA Polymerases, SYBR® Green dye, highest quality dNTPs, and buffer components at optimal concentrations. Hot Start Taq DNA Polymerases in the master mix provide antibody mediated hot start mechanism which releases more active enzymes and requires shorter activation time. SYBR® Green dye emits fluorescence when bound to double-stranded DNA. Detection of PCR product is monitored by the increase in fluorescence, leading to high sensitivity, wide dynamic range and high reproducibility for quantification.
ViPrimePLUS AtTaq qPCR Green Master Mix I can be used to amplify any DNA template including genomic, cDNA and viral sequences. The improved formulation of qPCR green master mix can detect extremely low copy number targets very specifically with high efficiency. The qPCR green master mix is designed to prevent and reduce the formation of primer dimers and non-specific products leading to optimum sensitivity and specificity.
ViPrimePLUS AtTaq qPCR Green Master Mix I has several formulations optimized to be used with most of real-time PCR instruments. The improved sensitivity and consistency of ViPrimePLUS AtTaq qPCR Green Master Mix I in standard cycling conditions gives the industry leading performance in fast cycling conditions.
Applications
All kinds of sample material suited for qPCR amplification can be used.
Features
- Ready-to-use real-time PCR reaction set up
- Rapid extension rate for early Ct values
- Contain Hot Start Taq DNA Polymerase - highest sensitivity and specificity
- Includes SYBR® Green for intercalator-based qPCR
- Increased limit of detection
- Compatible with most of the real-time PCR platforms
Component
1.6ml aliquots of master mix
Storage
Stable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.
Quality Control
As part of the ISO9001:2008 quality assurance systems, each lot of ViPrimePLUS AtTaq qPCR Green Master Mix I has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.
Limitation Of Use
For research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.
Instruments
To calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.
| Product Description | Compatible Hardware |
| QLMM02 ViPrimePLUS AtTaq qPCR Green Master Mix I (SYBR® Green Dye) |
Analytik Jena qTower series, BioRad iCycler all series, BioRad CFX96 & CFX384, Cepheid SmartCycler®, Eppendorf Mastercycler series, Fluidigm BioMark™, Illumina Eco, MJ Chromo4, Opticon, PCRMax Eco™, Roche lightcycler® series, Qiagen RotorGene, Thermo PikoReal™ |
| QLMM02-LR ViPrimePLUS AtTaq qPCR Green Master Mix I with Low ROX (SYBR® Green Dye) |
Agilent / Stratagene MX MX3000P®, MX3005P®, MX4000®, Applied Biosystems 7500 and 7500 FAST platform, QuantStudio™, ViiA7 |
| QLMM02-R ViPrimePLUS AtTaq qPCR Green Master Mix I with ROX (SYBR® Green Dye) |
Applied Biosystems 7000,7300,7700,7900 and 7900HT FAST platforms, OpenArray PRISM 7000,7700,7900, GeneAmp® 5700, StepOne™, StepOne™ PLUS |
Ordering Information
| Catalog No | Description | Pack Size |
| QLMM02 | ViPrimePLUS AtTaq qPCR Green MasterMix I (SYBR® Green Dye) | 150 reactions |
| QLMM02-LR | ViPrimePLUS AtTaq qPCR Green MasterMix I with Low ROX (SYBR® Green Dye) | 150 reactions |
| QLMM02-R | ViPrimePLUS AtTaq qPCR Green MasterMix I with ROX (SYBR® Green Dye) | 150 reactions |
Download
Manual
ViPrimePLUS AtTaq qPCR Green MasterMix I (SYBR® Green Dye)
ViPrimePLUS AtTaq qPCR Green MasterMix I with Low ROX (SYBR® Green Dye)
ViPrimePLUS AtTaq qPCR Green MasterMix I with ROX (SYBR® Green Dye)
Download
MSDS
ViPrimePLUS AtTaq qPCR Master Mix
