Description
The pTG19-T vector is designed for rapid and efficient cloning of PCR products with 3’dA overhangs. The linearized pTG19-T vector with 3’-dT overhangs prevent vector recircularization, therefore resulting in high percentage of recombinant clones and low background.
Features
- Convenient – ready-to-use linearized 3’dT overhang pTG19-T vector.
- Efficient –more than 80% of the recombinant clones contain the target DNA
- Rapid clone selection:
-lacZ gene for blue/white selection.
-M13 primer sites for PCR screening and sequencing.
-BamHI restriction enzyme can be used to release the insert from the pTG19-T vector.
Quality Control
- More than 80% clones are white with control insert.
- More than 85% of white clones are positively by restriction endonuclease digestion.
- The 3’dT overhangs for every batch vectors is confirmed by sequencing of five recombinant clones.
Kit Components
- pTG19-T vector
- Control insert
Storage & Stability
- All components are stable at -20°C for one year if properly stored.
- To avoid frequent feeze-thaw cycles, keeping small aliquots at -20°C is recommended.
Ordering Information
| Catalog No | Description | Pack Size |
|---|---|---|
| TA010 | pTG19-T Cloning Vector | 20 applications |
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Manual
pTG19-T Cloning Vector
Download
MSDS
pTG19-T Cloning Vector
Publication
This Product Has Been Used In:
Dadgar Pakdel et al. (2020) Identification, molecular characterization and expression of aminopeptidase N-1 (APN-1) from Anopheles stephensi in SF9 cell line as a candidate module for developing a vaccine that interrupt malaria transmission, Malaria Journal, 19:79.
Farahani S et al. (2020) Differential expression of heat shock proteins and antioxidant enzymes in response to temperature, starvation,and parasitism in the Carob moth larvae,. Ectomyelois ceratoniae (Lepidoptera: Pyralidae).PLoS ONE 15(1): e0228104.
Gosalawit C, Imsoonthornruksa, S, Udomsil, N & Ketudat-Cairns M (2020) Genome sequence of the oleaginous yeast Rhodotorula paludigena strain CM33, a potential strain for biofuel production. Microbiology Resource Announcement, 9:e00286-20
Nili O et al. (2020) Development of an efficient tef-1α RNA hairpin structure to efficient management of lasiodiplodia theobromae, Research Square 2
Mahboudi et al. (2018) Prospect and Competence of Quantitative Methods via Real-time PCR in a Comparative Manner: An Experimental Review of Current Methods, . The Open Bioinformatics Journal,11:1-11.
Abbaszadeh-Goudarzi, K., Abbaszadeh-Goudarzi, G., Khorramizadeh, M.R., Bouzari, S., Rezaie, S., Davari, M., Siadat, S.D., Teimoori-Toolabi, L. (2017). HMW1555-914, HMW2553-916, and Hia585-705 as Subunit Vaccine Candidates of Nontypeable Haemophilus influenzae Induce Specific Antibody Responses with Bactericidal Activity in Balb/c, . Journal of Microbiology, Vol, 11, No. 1 (2017).
Amini, S., Maali-Amiri, R., Mohammadi, R., Kazemi-Shahandashti,S.S. (2017) cDNA-AFLP analysis of transcripts induced in chickpea plants by TiO2 nanoparticles during cold stress. Plant Physiology and Biochemistry. 111. Pp39-49
Ayudhaya P.T.N., Muangmai N., Banjongsat N., Singchat W., Janekitkarn S., Peyachoknagul S., Srikulnath K. (2017), Unveiling cryptic diversity of the anemonefish genera Amphiprion and Premnas (Perciformes: Pomacentridae) in Thailand with mitochondrial DNA barcodes, In Agriculture and Natural Resources, Volume 51, Issue 3, Pages 198-205
Busayapongchai, P., Siri, S.(2017). Sensitive detection of estradiol based on ligand binding domain of estrogen receptor and gold nanoparticles. Analytical biochemistry. 518, pp.60-68.
Suntronpong, A., Thapana, W., Twilprawat, P., Prakhongcheep, O., Somyong, S., Muangmai, N., Peyachoknagul, S., Srikulnath, K. (2017). Karyological characterization and identification of four repetitive element groups (the 18S – 28S rRNA gene, telomeric sequences, microsatellite repeat motifs, Rex retroelements) of the Asian swamp eel (Monopterus albus), Comparative Cytogenetics, Vol. 11, No. 3, 435-462 (2017).
Kohnehrouz, B.B., & Nayeri, S. (2016) Design, Cloning and In silico Analysis of Efficient siRNA-inducing Casette for Silencing Wheat γ-gliadins Jordan Journal of Biological Sciences, 9(1), p.35-40.
Laopichienpong, N., Muangmai, N., Supikamolseni, A.,Twilprawat, P., Chanhome L., Suntrarachun, S., Peyachoknagul, S., Srikulnath, K. (2016). Assessment of snake DNA barcodes based on mitochondrial COI and Cytb genes revealed multiple putative cryptic species in Thailand. . Gene. 594(2).pp.238-247.
Rasoulinejad, S., Gargari, S.L.M. (2016) ) Aptamer-nanobody based ELASA for specific detection of Acinetobacter baumannii isolates. Journal of Biotechnology. 231. Pp.46-54
Uttatree,S., Charoenpanich, J. (2016) Isolation and characterization of a broad pH- and temperature-active, solvent and surfactant stable protease from a new strain of Bacillus subtilis. . Biocatalysis and Agricultural Biotechnology. 8. Pp.32-38
Meidaninikkjeh, S., Vaziri,F., Siadat, S.D. (2015) Cloning of conserved regions of nontypeable Haemophilus influenzae hmw1 core binding domain. International Journal of Molecular and Clinical Microbiology.5(1) pp.510-515
Nasanit, R., et al (2015) Assesment of Epiphytic Yeast Diversity in Rice (Oryza sativa) Phyllospehere in Thailand by a Culture-independent Approach Antonie van Leeuwenhoek.Springer. 107(6), p.1475-1490
Sasithorn Uttatreea, Jittima Charoenpanich (2015). Isolation and Characterization of a Broad Range pH and Temperature Active Protease from Staphylococcus saprophyticus. Burapha University International Conference 2015
Valadan, R., Amjadi, O., Tehrani, M., Rafiei, A., Hedayatizadeh-Omran, Alizadeh-Navaei, R. (2015) Pseudogene-free amplification of HPRT1 in quantitative reverse transcriptase polymerase chain reaction. Analytical biochemistry. 485. Pp.46-48
Valadan, R., Hedayatizadeh-Omran, A., Alhosseini-Abayazani, M.N., Amjadi, O., Rafiei, A., Tehrani, M., Alizadeh-Navaei, R., (2015) Data supporting the design and evaluation of a universal primer pair for pseudogene-free amplification of HPRT1 in real-time PCR. Data in brief. 4. Pp.384-389.
Babaei, S., Talebi, M., Bahar, M. (2014) Developing an SCAR and ITS reliable multiplex PCR-based assay for safflower adulterant detection in saffron samples. Food Control. 34. Pp..323-328
