Description
The ViPrimePLUS Foot & Mouth Disease Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Foot & Mouth Disease Virus genome in blood, vesicles and body fluid samples.
Foot and Mouth Disease is a contagious disease that on rare occasions infects humans but it is mostly prevalent among cloven-hooved livestock. The responsible pathogen, FMDV is a non-enveloped, single stranded RNA virus that is subdivided into 7 serotypes. Outbreaks from a particular serotype are usually regional in nature; with the exception of serotype O, which is the most common type of FMDV virus. Transmissions could be via airborne, skin or sexual contact, and ingestion of contaminated feed as the virus is present in all secretions and excretions of infected animals. Symptoms of the disease include fever and the presence of vesicles (blisters) on the feet, in and around the mouth and on the mammary gland. Infection could persist from 4 months to 4 years depending on the host. The result of reduced milk production, mastitis, abortion and weight losses in infected adult herds and the high mortality rate among the infected young animals are a major economical concern. Therefore, a convenient yet reliable screening routine is a good measure in disease management.
ViPrimePLUS Foot & Mouth Disease Virus RT-qPCR Kit is intended for research use only.
Kit Component
- FMDV Primers and Probe Mix (FMDV PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Ruminant samples (e.g. blood, vesicles, body fluid samples) |
| Gene Target | Outer 5’ untranslated region |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV6014 | ViPrimePLUS Foot & Mouth Disease Virus RT-qPCR Kit | 150 reactions |
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