Description
The ViPrimePLUS Feline Leukaemia Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Feline Leukaemia Virus genome in blood and bodily fluid samples.
Feline Leukaemia Virus (FeLV) is an oncogenic retrovirus that infects cats and causes leukaemia. It is often found in cats positive for feline immunodeficiency virus (FIV). There are 4 subgroups of FeLV; 1) Subgroup A which is found in naturally infected cats and tends to be less pathogenic, 2) Subgroup B which increases neoplastic development frequency, 3) Subgroup C which accounts for 1% of the infected cats, causes severe anemia and 4) Subgroup T which leads to lymphoid depletion and immunodeficiency. It is believed that subgroups C and D arise as the mutated form of subgroup A. The virus may be transmitted vertically (in utero or by milk) or horizontally (by secretions and excretions). Initial infection may be limited to lymphoid tissue but as second stage progresses, the virus may be circulated to the bone marrow, oesophagus, bladder, respiratory tract and salivary glands. Once infection reaches the bone marrow, mortality rates can raise up to 50-80%. The disorders due to the FeLV infections include immunosuppression, lymphoid tumour, anemia, reproduction problems and enteritis.
ViPrimePLUS Feline Leukaemia Virus RT-qPCR Kit is intended for research use only.
Kit Component
- FeLV Primers and Probe Mix (FeLV PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Feline samples (e.g. blood, body fluid) |
| Gene Target | U3 region LTR |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV2010 | ViPrimePLUS Feline Leukaemia Virus RT-qPCR Kit | 150 reactions |
Download
Manual
