Description
The ViPrimePLUS Mycoplasma haemofelis and haemocanis qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Mycoplasma haemofelis and Mycoplasma haemocanis genome in conjunctiva and blood samples.
Mycoplasmas are Gram negative microorganisms that have been isolated from many animal species. M. haemofelis, previously known as Haemobartonella felis, infects cats while M. haemocanis infects dogs, causing massive bacteremia of red blood cells that eventually leads to severe haemolytic anaemia. Both parasites are very similar genetically. They are predominantly transmitted via fleas and ticks bites. Clinical signs include anorexia, depression, lethargy, weakness, fever and weight loss. M. felis infected cats may remain a life-long carrier and infection can re-emerged through stress or immunosuppression. Similarly to M. haemocanis infected dogs where they will be long term carriers, however, symptoms will only recur in immunocompromised animals or after splenectomy.
ViPrimePLUS Mycoplasma haemofelis and haemocanis qPCR Kit is intended for research use only.
Kit Component
- M. haemofelis/haemocanis Primers and Probe Mix (M. haemofelis/haemocanis PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Canine and feline samples (e.g. conjunctiva, blood) |
| Gene Target | 16S ribosomal RNA gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV2014 | ViPrimePLUS Mycoplasma haemofelis and haemocanis qPCR Kit | 150 reactions |
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