Description
The ViPrimePLUS Mycoplasma haemofelis qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Mycoplasma haemofelis genome in conjunctiva and blood samples.
Mycoplasmas are Gram negative microorganisms that have been isolated from many animal species. M. haemofelis, previously known as Haemobartonella felis, can cause massive bacteremia of red blood cells that eventually leads to severe haemolytic anemia in cats. The pathogen is transmitted vertical from mother to offsprings and horizontally via fleas and ticks bites. The infection results in several diseases in cats, i.e. feline conjunctivitis, lower respiratory tract infections, feline infectious peritonitis and feline infectious anaemia (FIA). Recovered cats may remain a life-long carrier and infection can re-emerged through stress.
ViPrimePLUS Mycoplasma haemofelis qPCR Kit is intended for research use only.
Kit Component
- M. haemofelis Primers and Probe Mix (M. haemofelis PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Feline samples (e.g. conjunctiva, blood) |
| Gene Target | Heat shock protein 70 (dnaK) gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV2013 | ViPrimePLUS Mycoplasma haemofelis qPCR Kit | 150 reactions |
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Manual
