Description
The ViPrimePLUS Anaplasma phagocytophilum qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Anaplasma phagocytophilum genome in blood samples.
Anaplasmosis is an infectious, non-contagious disease that affects humans and ruminants, such as cattle, bison, sheep, goats, deer and elk. The transmission is usually tick-borne or via blood transfusion. The infection occurs primarily in warm tropical and subtropical areas, where the ticks thrive. There are several species which are well-recognized, for example the Anaplasma marginale and Anaplasma centrale which cause bovine anaplasmosis, Anaplasma ovis which causes ovine/caprine anaplasmosis, and Anaplasma phagocitophillum which is responsible for human granulocytic anaplasmosis and also infection in dogs, ruminants, horses and sheep. Erythrocytic anaplasma such as A. marginale, A. central and A. ovis infect the red blood cells and induce hemolysis, which causes anaemia. Among the symptoms are fever, depression, dehydration, difficulty in breathing, anaemia and possibly, death. However, most young calves rarely develop symptoms. Infected cattle remain as life-long carriers of the pathogens. On the other hand, A. phagocitophilum infects the neutrophils and circulates the host bloodstream. It may cause breathing difficulty, haemorrhage, renal failure or neurological problems where immunocompromised patients are more susceptible to more severe complications. Giemsa stain is still the commonly used diagnostic procedure although it lacks sensitivity and requires high expertise. Therefore, molecular diagnostics can be a more sensitive and specific alternative.
ViPrimePLUS Anaplasma phagocytophilum qPCR Kit is intended for research use only.
Kit Component
- A. phagocytophilum Primers and Probe Mix (A. phagocytophilum PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | • Human clinical samples (e.g. blood)< br />• Animal samples (e.g. ruminants) |
| Gene Target | Major surface protein 4 (msp4) gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV1001 | ViPrimePLUS Anaplasma phagocytophilum qPCR Kit | 150 reactions |
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