Description
The ViPrimePLUS Beak and Feather Disease Virus qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Beak and Feather Disease Virus (BFDV) genomes in blood and feather samples.
Beak and Feather Disease also known as the psittacine circoviral disease, is a highly lethal disease affecting mainly parrots and related species. The beak and feather disease virus is a DNA virus, which attacks the feather and beak cells. Symptoms of disease include feather loss, abnormal feather coloring, deformed feather and beak (longer and more brittle beak), diarrhoea, vomiting and weight loss. In some advanced cases, necrosis in the hard palate leads to difficult in eating. The virus also causes immunosuppression and younger birds are more susceptible to the infection. Birds that survived through the initial infection will become chronic shredder of the virus and are highly contagious. Currently, there are no effective treatment and vaccine for this disease.
ViPrimePLUS Beak and Feather Disease Virus qPCR Kit is intended for research use only.
Kit Component
- BFDV Primers and Probe Mix (BFDV PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Avian samples (blood, feather) |
| Gene Target | Putative replication-associated protein (ORF1) gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV1009 | ViPrimePLUS Beak and Feather Disease Virus qPCR Kit | 150 reactions |
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Manual
