Description
The ViPrimePLUS Gallid herpesvirus 2 qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Gallid herpesvirus 2 genome in blood, skin scabs and organ tissues.
Gallid herpesvirus 2 (GaHV-2) is a double-stranded DNA virus which causes Marek’s disease in chickens. This common malignant disease is highly contagious and spreads by bird to bird contact, contact with infected dust and dander and aerosol inhalation. It is accountable for a substantial growth defects and deaths in chickens, resulting in major economically losses in the poultry industry. There are 4 forms of Marek’s presentation; 1) Cutaneous, 2) Neural, 3) Ocular, 4) Visceral. The disease is characterized by the presence of T cell lymphoma in different forms, giving rise to limbs paralysis, immunosuppression, and lesions on feather follicles, breathing difficulty, gray irises or irregular pupil, blindness and tumor formation in the internal organs. The latent infection is responsible for the long term carrier status of the recovered birds.
ViPrimePLUS Gallid herpesvirus 2 qPCR Kit is intended for research use only.
Kit Component
- GaHV-2 Primers and Probe Mix (GaHV-2 PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Avian samples (blood, skin cabs, tissues) |
| Gene Target | Meq gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included | • Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels | • FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV1017 | ViPrimePLUS Gallid herpesvirus 2 qPCR Kit | 150 reactions |
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